The study investigated the impact of varied levels of Psidium guajava leaf meal (PGLM) on internal, external, and biochemical egg qualities of hens exposed to diets contaminated by aflatoxin B (AFB ). Ninety-six point-of-lay Isa Brown pullets were randomly assigned to four dietary groups; each replicated six times with four birds per replicate in a completely randomized design (CRD). The experimental diets included: A (control), B (basal with 1 mg AFB /kg diet), C (Diet B with 5 g PGLM /kg diet), and D (Diet B with 10 g PGLM /kg diet). Among the internal egg qualities, albumen weight, yolk length, and yolk width were significantly reduced (P0.05). However, supplementation with 5 and 10 g PGLM/kg in Diets C and D, respectively, significantly improved these parameters. External egg qualities such as shell weight, thickness, ratio, and index were significantly affected (P<0.05), with reductions observed in birds fed Diet B. Biochemical analysis of yolk components indicated significant reductions (P<0.05) in SOD, CAT, GPx, TAC, and HDL-C levels, and increases (P<0.05) in MDA, TG, TC, and LDL-C levels in birds fed Diet B compared to Diet A. Diets C and D mitigated these adverse effects, showing significant improvement (P<0.05) across these parameters. Contamination of layer diets with 1 mg AFB /kg negatively impacts egg quality, while supplementation with 5 to 10 g PGLM /kg effectively mitigates these effects. Thus, incorporating PGLM into AFB-contaminated diets shows promise for maintaining egg quality in layers.