Cattle is one of the most domesticated livestock in Nigeria, but its production and consumption is affected by parasitic pathogens. This cross-sectional study was carried out to compare the gut parasites of cattle slaughtered in two abattoirs in Lagos State, Nigeria. Faecal samples obtained directly from the rectum of 132 slaughtered cattle were analyzed using formol-ether concentration technique. Out of the 132 samples examined, a total of 69 were infected with an overall prevalence of 52.3%. Three GIT helminth parasites were isolated namely; Fasciola sp. (52.3%), Strongyloides sp. (30.4%) and Eimeria sp. (17.4%). In Fatai Atere and Odo-Eran abattoirs, female cattle were the most infected 21(35.5%) and 17(37.4%) respectively (p=0.815). Based on study location, parasitic prevalence was higher in Fatai Atere abattoir 59.1% than in Odo-Eran abattoir 45.5% (p>0.05). Age, funds (inadequate feeding and watering space), farmer education and poor ventilation/ overcrowding were found to be risk factors for GIT parasite infection among cattle in the study areas (p<0.05). This study suggested that helminth infection is endemic in the region and hence, routine treatment, adequate sanitation and good management practices should be adopted to mitigate cases of parasitic helminth infection of cattle in the study areas.