The effect of sex on morphometric body measurements of donkeys in the Sahel agro-ecological zone of the north eastern Nigeria was assessed. A total of 200 adult donkeys were sampled through stratified random sampling in some parts of Borno and Yobe States and were used for the phenotypic study. Phenotypic body measurements obtained were subjected to ANOVA. Sexual dimorphism was pronounced only on body weight while only height at withers and neck length were the only morphometric measurements that demonstrated sexual dimorphism. There were significant differences (p<0.05) in body weight, body length, height at wither, ear length, and back length in donkeys assessed in Yobe State which had higher values than those in Borno State. The tail length, and the distance between ear and hind leg length were significantly different (p<0.05) in donkeys obtained in Borno State from those in Yobe State. Most of the traits were significantly affected (p<0.05) by location. The interaction effects of sex and location on bodyweight and morphometric body measurements of the donkeys showed that male was significantly different (p< 0.05) in those of Yobe State compared to those in Borno State. The interaction effects of sex and location revealed that most of the morphometric traits were higher in female. This might be due to interactions between sex and location, managements and environmental conditions. The morphometric body measurements of the donkeys in the Sahel agro-ecological zone of the North eastern Nigeria present diversities in location and sexual dimorphism.