The effects of supplementing roasted soyabean-based diets with the first two limiting amino acids (L-lysine and DL-methionine) on growth performance and proximate composition of Clarias gariepinus were investigated in this study. Juvenile fish (n=360), weighing 19.60±0.10g were randomly allocated to six diets containing varied supplemental combinations of lysine + DL-methionine (g/100g) in the formulated basal roasted soyabean-based diets (RSBD): R (without 1 supplemental lysine and DL-methionine), R (0+1g), R (0.25g+0.75g), R 2 3 4 (0.5g+0.5g), R (0.75g+0.25g), R (1g+0) of supplemental L-lysine + DL- 5 6 methionine, respectively. The fish were fed to satiation with the diets for 12 weeks. Each treatment was in triplicate. Results revealed that the diets had no significant influence (P>0.05) on fish final weight and feed conversion ratio. Gross protein retention of fish on R (1.01±0.04) and R (0.97±0.04) were similar. The crude 3 5 protein composition of whole-body C. gariepinus on R (70.03±1.60) and R 5 3 (68.39±1.46) were not significantly different (p>0.05) but significantly higher than those on other diets. Supplemental amino acids significantly increased the methionine composition (P<0.05) with the highest value of 2.42±0.04 in fish on R 4 and least value of 2.12±0.02 in those on R . Regression of crude protein of fish 2 whole body and supplemental lysine and methionine were both quadratic with the optimum dietary inclusion levels of 0.6g/100g (R2= 0.79) and 0.4g/100g (R2=0.76), respectively. In conclusion, dietary supplement of lysine and methionine in roasted soyabean-based diets had no impact on growth performance but enhanced the body composition of C. gariepinus.